Protein_Domain

Part:BBa_K1459011:Design

Designed by: Grzegorz Ścibisz   Group: iGEM14_Warsaw   (2014-10-07)


PmrB(N-term)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14


Design Notes

We had to truncate the sequence of PmrB just before start of iron binding tag on the extracellular loop of PmrB.


Source

We obtained PmrA-PmrB sequences thanks to kindness of prof. He of the University of Chicago.

References